APPLIED BIOANALYTICAL LABS
providing tools for oxidative stress research
Contract Assay Service
Our Services are focused on testing antioxidant and biomarkers related to oxidative damage in
samples ranging from plasma (serum), red blood cell, tissue biopsy, semen, wine, fruits, vegetables
and botanical extracts, for the purpose of
wellness monitoring.  

Our contract assay services are in four main categories:  1) Activities of antioxidant enzymes, 2) small molecule
biomarkers, 3) Total antioxidant capacities (excluding antioxidant enzymes, based on inhibition/retardation of
damage to a target molecule caused by free radicals), and 4) Direct observation of scavenging effect of
antioxidants on a specific free radical using electron spin resonance (EPR) spectroscopy.
Catalog numbers
Description    
Samples  
Unit Price*
Antioxidant Enzyme Assay Service
SOD
Superoxide Dismutase Acitivity
RBC, tissues
$169
GPx
Glutathione Peroxidase Activity
RBC, tissues
$169
GR
Glutathione Reductase Activity
RBC, tissues
$169
CAT
Catalase Activity
RBC, tissues
$169
Small Molecule Biomarker Assay Service (GSH Assay Service & TBARS Assay Service)
GSH
Total Glutathione Concentration
RBC, Tissues
$169
TBARS
Damaged Lipid Assessement
Plasma, tissues, fasted urine,
semen plasma
$169
Total Antioxidant Assay Service (Total Antioxidant Analysis Service)
TAC - Peroxyl
Total antioxidant capacity
against peroxyl radical based on
inhibition of damage to a target
molecule
plasma (serum), semen
plasma, tissues, wine, fruit,
vegetables, botanical extracts
$169
TAC - Peroxynitrite
Total antioxidant capacity
against peroxynitrite based on
inhibition of damage to a target
molecule
plasma (serum), semen
plasma, tissues
inquire
TAC - Hypochlorite
Total antioxidant capacity
against hypochlorite based on
inhibition of damage to a target
molecule
wine, tea, botanical extracts
inquire
Scavenging Capability of Specific Free Radicals
EPR - hydroxyl scavenging
Hydroxyl radical scavenging
capability based on EPR spin
trapping
botanical extracts, wine
inquire
EPR - superoxide
Scavenging
Superoxide scavenging
capability based on EPR spin
trapping
botanical extracts, wine
inquire
* Contact us if you need specific details and/or literature of our assay methods. A $39 extra sample processing
charge will be applied to each tissue sample submitted. Standard turnaround time is two weeks.  Extra charges
for *rush* work will apply. Discounts are available for quantities over 5 samples, and we also offer special pricing
for major projects.

Please fill out our Sample Submission Form, you must include this form with your sample shipment.   

Oxidative stress, antioxidant and wellness:
Reactive oxygen free radicals have been implicated in more than 100 human diseases and in aging process.  
Tissue damage caused by free radicals is also well documented in trauma, toxin shocks, ischemia/reperfusion.  
Following figure shows common endogenous and exogenous sources of reactive oxygen species (ROS).   
To prevent deleterious damage from ROS, human body has developed a powerful antioxidant defense system. It
includes enzymes such as superoxide dismutase, glutathione peroxidase and catalase to decompose these
harmful species, small free radical-scavenging molecules such as ascorbic acid, glutathione, uric acid, vitamin-E and
CoQ-10, and macromolecules to chelate metals and adsorb free radicals.  Other small molecules from diet and
nutritional supplements also exhibit strong antioxidant properties.  
Endogenous:

  • Aerobic Respiration
O2-    --->   H2O2 (~2% of total O2 respiration)     
  • Leukocytes/Macrophages (Inflammation)
O2-    --->   H2O2  HOCl  HOBr  OONO-
  • Peroxisomes
H2O2
  • P450 – Liver
O2-   R.   
  • Lipid Peroxidation Chain Reaction
(H2O2 + Fe/Cu  ----->  OH.)  ----->
(OONO-             ----->  OH.)  -----> ROO.
Exogenous:

  • Tobacco Smoking
  • Ionizing Radiation
  • UV Light Exposure
  • Pollutants (including ozone)
  • Organic Solvents
  • Anesthetics
  • Hyperoxic Environment
  • Pesticides
  • Some Medications